Serum free light chains

Quantitation of serum free light chains.

under usual artificial light conditions, and storage of unproc-essed whole blood without antico-agulants at room temperature on the bench. Additionally, we tested the effect of multiple freeze-thaw cycles. 25(OH)–vitamin D 3 measurements were performed on a Cobas E601 an-alyzer (Roche), using the vitamin D 3 (25-OH) assay as recommended by the manufacturer. Samples from each storage experiment ...

Measurement of immunoglobulin free light chains in serum.

but the active/latent isoform proportions were similar (Fig. 1A). Platelet aggregation during clotting (6) may have caused these differences. MMP expression was lower in K 2 E plasma than in LH plasma [14 (3) vs 25 (4) ␮g/L; P Ͻ0.05; Fig. 1A, lane 4 vs lane 5]. The concentrations of MMP-9 forms decreased significantly with increasing amounts of K 2 E during PB collection, whereas MMP-2 was incr...

Regarding the overestimation of serum κ free light chains.

Employment or Leadership: None declared. Consultant or Advisory Role: F.C. Wu, Organon International, Bayer Schering Pharma, Eli Lilly, Proctor & Gamble, Pierre Fabre Medicaments, Ferring Pharmaceuticals, TAP Pharmaceuticals, Ardana Bioscience, and GlaxoSmithKline. Stock Ownership: None declared. Honoraria: F.C. Wu, Organon International, Bayer Schering Pharma, Lilly ICOS, Ardana Bioscience, an...

Identification of amyloidogenic light chains requires the combination of serum-free light chain assay with immunofixation of serum and urine.

BACKGROUND The diagnosis of systemic immunoglobulin light-chain (AL) amyloidosis requires demonstration of amyloid deposits in a tissue biopsy and amyloidogenic monoclonal light chains. The optimal strategy to identify the amyloidogenic clone has not been established. We prospectively assessed the diagnostic sensitivity of the serum free light chain (FLC) kappa/lambda ratio, a commercial serum ...

Measurement of free light chains in urine.